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. 2012 Aug 31;7(8):e43778. doi: 10.1371/journal.pone.0043778

Figure 2. Cloning and functional analysis of a novel MMTV-tTA construct.

Figure 2

A. Schematic outline of the new transgene and its applicability to control the expression of TetO-promoter driven reporter genes. B. Bioluminescence imaging of HC11 cells co-transfected with a TetO-luciferase plasmid and the MMTV-tTA construct or a pMSCV-tTA vector as a positive control. Transfected cells were cultured for 96 hours in the absence or presence of Dox (1 µg/ml). Note that the addition of lactogenic hormones (DIP: Dexamethasone, Insulin, and Prolactin) enhances the expression of the MMTV-tTA and luciferase.