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. 2012 Aug 31;7(8):e43704. doi: 10.1371/journal.pone.0043704

Table 3. Effect of V3, MPER and IDR peptides on ID50 neutralization titers of the cross-neutralizing plasma in depletion and competition experiments.

aID50 bID50 fold decrease
Plasma ID Virus Untreated plasma Mock D V3 D V3+ MPER D MPER+ IDR D ID+ V3 D V3+ MPER D MPER+ IDR D ID+
AIIMS206 AIIMS201 >20000 10597 13143 >20000 8362 1370 17776 10891 0.806 1 1.2 14.5 0.59 1.83
AIIMS212 4022 3684 3965 3565 4345 862 3807 3123 0.929 1.12 0.84 4.66 0.96 1.28
Du156.12 3106 2398 2479 2172 2648 1903 2172 2479 0.967 1.43 0.9 1.63 1.1 1.25
ZM109F.PB4 3243 2737 2737 2924 2829 3123 2280 2924 1 1.1 0.96 1.03 1.2 1.1
ZM53M.PB12 17766 14259 12495 11697 8982 4641 7129 8670 1.14 1.51 1.58 3.82 2 2.04
JRFL 3040 2648 2829 3123 3123 3448 3456 3564 0.936 0.97 0.84 0.88 0.76 0.85
RHPA4259.7 3313 2829 1967 1724 2320 1967 2398 2400 1.43 1.92 1.21 1.68 1.17 1.38
TRO.11 4199 3564 2737 3228 2479 1281 2737 2102 1.3 1.3 1.43 3.27 1.3 1.99
AIIMS239 AIIMS201 783 543 508 476 390 476 640 731 1.06 1.64 1.39 1.64 0.84 1.07
AIIMS212 457 331 353 445 281 150 342 431 0.93 1.02 1.17 3.04 0.96 1.06
Du156.12 317 254 230 281 223 101 263 238 1.1 1.12 1.13 3.1 0.96 1.33
ZM109F.PB4 220 230 215 230 108 123 195 246 0.92 0.95 2.12 1.78 1.17 0.89
ZM53M.PB12 1257 1324 525 620 119 120 921 755 2.52 2.02 11.12 10.47 1.43 1.66
JRFL 245 290 281 223 140 78 290 223 1.03 1.09 2.07 3.14 1 1.09
RHPA4259.7 509 445 320 310 171 111 310 246 1.39 1.64 2.6 4.58 1.43 2.06
TRO.11 385 310 286 290 127 104 320 310 1.08 1.32 2.44 3.7 0.96 1.24
AIIMS249 AIIMS201 457 390 476 662 417 525 419 476 0.819 0.69 0.93 0.87 0.93 0.96
AIIMS212 520 525 640 492 290 230 640 862 0.82 1.05 1.81 2.26 0.82 0.6
Du156.12 189 170 166 159 156 106 149 140 1.02 1.18 1.08 1.78 1.14 1.35
ZM109F.PB4 189 223 177 230 171 119 140 160 1.25 0.82 1.3 1.58 1.59 1.18
ZM53M.PB12 369 342 342 340 353 202 353 365 1 1.08 0.96 1.82 0.96 1.01
JRFL 291 281 300 246 246 215 310 271 0.936 1.18 1.14 1.35 0.906 1.07
RHPA4259.7 120 111 115 115 123 119 111 108 0.965 1.04 0.9 1 1 1.11
TRO.11 734 755 707 662 755 599 731 720 1.06 1.1 1 1.22 1.03 1.01

The table describes the change in ID50 neutralization titers of the three cross-neutralizing plasma CNP (AIIMS206, AIIMS239 and AIIMS249) against 5 subtype-C and 3 subtype-B viruses upon depletion and competition with V3, MPER and IDR specific peptides.

a

ID50; The numerical values are the ID50 neutralization titers of CNP after depletion (‘D’) (passing plasma over antigen coated ELISA plates @10 µg/ml) and competition ‘+’ (incubating CNP with specific peptides @20 µg/ml) along with untreated and mock (passed CNP on uncoated ELISA plates) controls.

b

ID50 fold decrease; Plasma ID50 fold decrease for depletion assay was calculated as, ID50 with mock depleted plasma/ID50 with V3, MPER and IDR depleted plasma, and for competition assay as ID50 with untreated plasma/ID50 with V3, MPER and IDR treated plasma. The more than threefold change in ID50 neutralization titer was arbitrarily taken as positive. The CNP AIIMS206 and AIIMS239 showed dependence on MPER (Bold) directed antibodies especially with peptide competition while V3 and IDR showed minimal effect on neutralization as compared to untreated and mock plasma controls.