Table 1. Trapped endogenous genes from G418-resistant HeLa cells.
Junction sequences | Gene name | Accession number | Chromosomename | Exon number | Insertion sites |
gtgtctctcctatcTA cagttgaag | IPNN5B | NM_005540.2 | Chr:1 | 18 | intron1 |
ttggattttatacaTA cagttgaag | APAF1 | NG_029094.1 | Chr:12 | 27 | intron16 |
aaactctttacataTA cagttgaag | HERC2 | NM_004667.4 | Chr:15 | 93 | intron66 |
ccctttttgttaacTA cagttgaag | MPP2 | NM_005374.3 | Chr:17 | 13 | intron3 |
tctctttcacaaacTA cagttgaag | TRIO | NT_006576.16 | Chr:5 | 57 | intron2 |
tgacagaacatagaTA cagttgaag | C4orf32 | NM_152400.2 | Chr: 4 | 2 | intron1 |
atacatcttatagtTA cagttgaag | ABCB11 | NG_007374.1 | Chr: 2 | 15 | intron5 |
gcacccactgtacaTA cagttgaag | CPZ | NM_001014447.2 | Chr:4 | 11 | intron3 |
tccaacaccacatcTA cagttgaag | CACNA1E | NM_001205293.1 | Chr:1 | 48 | intron6 |
agaatattctgcatTA cagttgaag | RP11-553P9.3 | NT_016354.19 | Chr:4 | 5′UTR | |
gttccttcccaaccTA cagttgaag | GRXCR1 | NG_027718.1 | Chr:4 | 4 | intron1 |
tagcattggggagcTA cagttgaag | U6snRNA | NW_001838848.1 | Chr: 2 | N/A | N/A |
tttataatgacttaTA cagttgaag | TMEM26 | NM_178505.6 | Chr:10 | 7 | intron1 |
tgaggggaaaaataTA cagttgaag | TPST2 | NM_001008566.1 | Chr:22 | 7 | exon7 |
tactatcttgttacTA cagttgaag | IMMT | NM_006839.2 | Chr:2 | 15 | intron3 |
tctaagaattcaccTA cagttgaag | RPL2I | NT_024524.14 | Chr:13 | 6 | intron1 |
ttcagctgagtacaTA cagttgaag | Unknown | AL139023.6 | Chr:14 | N/A | N/A |
taaaatcaatcttaTA cagttgaag | DENNEB | NM_144977.4 | Chr: 1 | 20 | intron17 |
Junction sequences were obtained by the splinkerette PCR. Partial trap cassette sequences in italic were shown on the right of TA and partial genome sequences in regular on the left of TA.