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. 2012 Aug 31;7(8):e44177. doi: 10.1371/journal.pone.0044177

Figure 4. Quantification of nuclear aggregate formation.

Figure 4

Vero cells were infected with 10 PFU/cell of HSV1(17+)blueLox (B) or HSV1(17+)blueLox-GFPVP26Δaa1–7-gDmRFP (C), and the cells were fixed at 4, 6, 8, 10, or 12 h. After permeabilization, HSV1(17+)blueLox infected cells were labeled with an antibody directed against VP26. According to their intranuclear VP26 phenotype, the cells were classified into “none”, “single”, “grainy” and “aggregate” (A). The numbers above the columns describe the number of nuclei analyzed for each time point.