Figure 3. Glycogen accumulation during acid and alkaline pH stress.

(A) Glycogen content in the wild-type strain. Glycogen was extracted from mycelia grown under physiological conditions (pH 5.8, control) and under acid (pH 4.2) and alkaline (pH 7.8) stress. After 120 min, the remaining cultures were transferred back to physiological conditions (pH 5.8, RE, recuperation). The results shown are the average of at least three independent experiments. (B) Glycogen content in the pacC^KO strain compared to the wild-type strain. 0, cell samples before the pH shift (control).