Figure 1. Multiple alignments of 17 deduced amino acid sequences of putative trypsins and trypsin homologs from O. nubilalis larvae using Clustal W2.

The predicted signal peptide is underlined in brown; the catalytic triads and conserved regions are boxed in blue; the conserved catalytic triads are marked with blue arrows at the top; the autocatalytic site is marked with green arrow at the top; the conserved residue in the S1 pocket (or trypsin-determination residue) is marked with purple arrow at the top; and the six cysteine residues were marked with purple stars at the top.