Figure 5. Primary histiocytic sarcomas are serially transplantable in NOD.SCID mice.

(A) Schematic diagram showing experimental design for secondary transplantation. GFP⁺ cells were sorted from freshly isolated primary tumor, induced initially by transplantation of gene-modified bone marrow cells into the cardiotoxin pre-injured muscle of NOD.SCID recipients, and injected into gastrocnemius muscle of secondary NOD.SCID recipients at different cell dosages (100 000, 10 000, 2 000, or 200 cells) following cardiotoxin pre-injury. (B) H & E staining of tumor cells (60x) in skeletal muscle of a secondary recipient. Immunohistochemical staining of secondary tumors shows positive staining for the myeloid marker Mac2, and negative staining of B220 and Ter119 (40x). (C) H & E staining of liver and spleen from secondary recipient mouse, showing aggressive infiltration of myeloid leukemia cells (60x).