Skip to main content
. 2012 Aug 31;7(8):e44855. doi: 10.1371/journal.pone.0044855

Figure 4. Immunofluorescence, Western blot and RT-PCR of CRYAA, HSP70 and PRDX6.

Figure 4

Immunofluorescence (A), western blot (B) and RT-PCR (C) results of CRYAA, HSP70 and PRDX6 in retinas of wide-type, untreated rd12 and treated rd12 eyes at P42. Data are a composite of 3 independent experiments (for A, B and C), and n = 6/group (B) and 4/group (C). The green staining in A represents the targeted proteins (FITC) while the blue color indicates nucleus staining with DAPI. Western blot (B) confirmed that all 3 proteins had higher expressions in untreated rd12 group, while the difference between the treated rd12 and C57BL/6J group was not significant. RT-PCR results (C) showed that CRYAA mRNA level was down-regulated in all 3 groups, and the untreated rd12 group demonstrated the lowest levels. HSP70 mRNA levels of the untreated rd12 group were 2 times higher compared to the treated rd12 group (p<0.05), and 1.6 times higher compared to the C57BL/6J group (p>0.05). PRDX6 mRNA of the untreated rd12 group was 2.4 times higher compared to the other 2 groups (p<0.05), while the mRNA levels of the treated rd12 group were not different compared to that of the C57BL/6J group. Error bars depict the standard error of the mean. Calibration bar  = 50 μm.