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. 2012 Aug 31;7(8):e44855. doi: 10.1371/journal.pone.0044855

Figure 5. Reactive oxygen species and apoptosis determination at age P42.

Figure 5

Green fluorescent staining indicates expression of ROS in the upper row panels, while it indicates apoptotic cell death by TUNEL in the lower row panels. Data are a composite of 3 independent experiments. Note the dramatic decrease in ROS presence in treated rd12 eyes (right upper panel) compared to the abundant presence of ROS in the untreated rd12 eyes (middle upper panel). Similarly, no signs of apoptosis are present in the treated rd12 eyes (lower right panel), and several apoptotic cells could be identified in the untreated rd12 eyes (middle lower panel). Calibration bar  = 50 μm.