Table 1.

Inhibition of human sEH of 4-(3-adamantan-1-yl-ureido)-butyric acid derivatives.

Compound	R	Human sEH IC50 (nM)a
3	H	684
4		10
5		4
6		4
7		12
8		67
9		4

^aHuman sEH (0.96 nM) was incubated with inhibitors for 5 min in 25 mM Bis-Tris/HCl buffer (200 μL; pH 7.0) at 30°C before fluorescent substrate introduction ([S] = 5 μM). Results are averages of three separate measurements. The fluorescent assay as performed here has a standard error between 10 and 20%, suggesting that differences of 2-fold or greater are significant.