Table 2.
Inhibition of human sEH of 3-(3-adamantan-1-yl-ureido)-cyclohexylcarboxylic acid derivatives.
| Compound | Structure | Human sEH IC50 (nM)a |
|---|---|---|
| 10 |
|
1160 |
| 11 |
|
2 |
| 12 |
|
3 |
| 13 |
|
9 |
a
Human sEH (0.96 nM) was incubated with inhibitors for 5 min in 25 mM Bis-Tris/HCl buffer (200 μL; pH 7.0) at 30°C before fluorescent substrate introduction ([S] = 5 μM). Results are averages of three separate measurements. The fluorescent assay as performed here has a standard error between 10 and 20%, suggesting that differences of 2-fold or greater are significant.