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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Free Radic Biol Med. 2012 Jun 6;53(5):1123–1138. doi: 10.1016/j.freeradbiomed.2012.05.036

Figure 11. MitoQ and Mito-CP treatment attenuates the I/R-induced increased adhesion molecule expression and enhanced delayed neutrophil infiltration in the liver.

Figure 11

Figure 11

Panel A: Real-time PCR shows significant increase in hepatic adhesion molecule ICAM-1 mRNA level at 2h of reperfusion (I/R 2h), and a gradual decrease by 24 hours (I/R 24h). Pretreatment with MitoQ/Mito-CP (3 mg/kg i.p.) significantly attenuates the I/R-induced increased level of adhesion molecule. n=7–12/group. *P<0.05 sham control vs. I/R; #P<0.05 I/R vs. corresponding I/R+MitoQ/Mito-CP.

Panel B: Quantification of MPO activity from the liver extracts shows significant time-dependent increases associated with I/R (peaking at I/R 24h), which are attenuated by pretreatment with MitoQ/Mito-CP (3 mg/kg i.p.). n=8–12/group. *P<0.05 sham control vs. I/R; #P<0.05 I/R vs. corresponding I/R+MitoQ/Mito-CP.

Panel C–D: Myeloperoxidase (MPO) staining (brown) of representative liver sections of sham mice treated with vehicle (sham) or MitoQ/Mito-CP, and mice exposed to 1 hour of ischemia followed by 2, 6 or 24 hours of reperfusion treated with vehicle or MitoQ/Mito-CP. These images reveal increased neutrophils attachment to the endothelium and accumulation in the vessels at I/R 6h followed by marked infiltration of liver tissue at I/R 24h. Pretreatment with MitoQ/Mito-CP (3 mg/kg i.p.) attenuates the I/R-induced increased neutrophil infiltration. Slides were counterstained by nuclear fast red. Panel C images depict 200× magnification, while Panel D depicts 400× magnification. A similar histological profile was seen in three to five livers/group.