Fig. 9.

Representative fluorescent micrographs showing comparison of surfaces of NO releasing and control films after incubation for seven days at 37 °C in a CDC biofilm reactor containing S. aureus or E. coli. The NO releasing films containing a base layer of 30 wt% DBHD/N₂O₂ mixed with a-50-50, e-50-50, e-75-25 or e-PLL PLGA matrix, and the NO release profile of the films are shown in Fig. 4B. The controls include two films with base layers of a-50-50 PLGA or a-50-50 PLGA mixed with 30 wt% DBHD and a film with only a SR layer. Bacterial cells were stained with Bacterial LIVE/DEAD staining dyes and viable cells shown as green fluorescent dots while dead or membrane damaged cells shown as red fluorescence dots in the images.