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. 1977 Nov;4(11):4015–4022. doi: 10.1093/nar/4.11.4015

Repair of ionizing radiation induced DNA damage in human lymphocytes.

M F Lavin, C Kidson
PMCID: PMC343218  PMID: 593898

Abstract

Phytohemagglutinin stimulated human lymphocytes exhibit a 20 fold increase in DNA repair synthesis following ionizing radiation damage compared to the level of repair in unstimulated cells. The peak of repair synthesis coincides with that for DNA replication. Stimulated lymphocytes provide a relatively simple assay for ionizing radiation repair defects.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson R. E., Warner N. L. Ionizing radiation and the immune response. Adv Immunol. 1976;24:215–335. doi: 10.1016/s0065-2776(08)60331-4. [DOI] [PubMed] [Google Scholar]
  2. Anderson R. E., Warner N. L. Radiosensitivity of T and B lymphocytes. III. Effect of radiation on immunoglobulin production by B cells. J Immunol. 1975 Jul;115(1):161–169. [PubMed] [Google Scholar]
  3. Bender M. A., Brewen J. G. Factors influencing chromosome aberration yields in the human peripheral leukocyte system. Mutat Res. 1969 Sep-Oct;8(2):383–399. doi: 10.1016/0027-5107(69)90016-5. [DOI] [PubMed] [Google Scholar]
  4. Bertazzoni U., Stefanini M., Noy G. P., Giulotto E., Nuzzo F., Falaschi A., Spadari S. Variations of DNA polymerase-alpha and -beta during prolonged stimulation of human lymphocytes. Proc Natl Acad Sci U S A. 1976 Mar;73(3):785–789. doi: 10.1073/pnas.73.3.785. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cleaver J. E. Defective repair replication of DNA in xeroderma pigmentosum. Nature. 1968 May 18;218(5142):652–656. doi: 10.1038/218652a0. [DOI] [PubMed] [Google Scholar]
  6. Conard R. A. Quantitative study of radiation effects in phytohaemagglutinin-stimulated leukocyte cultures. Int J Radiat Biol Relat Stud Phys Chem Med. 1969;16(2):157–165. doi: 10.1080/09553006914551171. [DOI] [PubMed] [Google Scholar]
  7. Drewinko B., Humphrey R. M., Trujillo J. M. The radiation response of a long-term culture of human lymphoid cells. I. Asynchronous populations. Int J Radiat Biol Relat Stud Phys Chem Med. 1972 Apr;21(4):361–373. doi: 10.1080/09553007214550431. [DOI] [PubMed] [Google Scholar]
  8. Edenberg H., Hanawalt P. Size of repair patches in the DNA of ultraviolet-irradiated HeLa cells. Biochim Biophys Acta. 1972 Jul 20;272(3):361–372. doi: 10.1016/0005-2787(72)90389-9. [DOI] [PubMed] [Google Scholar]
  9. German J. Genes which increase chromosomal instability in somatic cells and predispose to cancer. Prog Med Genet. 1972;8:61–101. [PubMed] [Google Scholar]
  10. Grossman L., Braun A., Feldberg R., Mahler I. Enzymatic repair of DNA. Annu Rev Biochem. 1975;44:19–43. doi: 10.1146/annurev.bi.44.070175.000315. [DOI] [PubMed] [Google Scholar]
  11. Han T., Pauly J. L., Minowada J. In vitro preferential effect of irradiation on cultured T lymphoid cell line. Clin Exp Immunol. 1974 Jul;17(3):455–462. [PMC free article] [PubMed] [Google Scholar]
  12. Kataoka Y., Sado T. The radiosensitivity of T and B lymphocytes in mice. Immunology. 1975 Jul;29(1):121–130. [PMC free article] [PubMed] [Google Scholar]
  13. Lieberman M. W., Sell S., Farber E. Deoxyribonucleoside incorporation and the role of hydroxyurea in a model lymphocyte system for studying DNA repair in carcinogenesis. Cancer Res. 1971 Sep;31(9):1307–1312. [PubMed] [Google Scholar]
  14. Loeb L. A., Ewald J. L., Agarwal S. S. DNA polymerase and DNA replication during lymphocyte transformation. Cancer Res. 1970 Oct;30(10):2514–2520. [PubMed] [Google Scholar]
  15. Mayer R. J., Smith R. G., Gallo R. C. DNA-metabolizing enzymes in normal human lymphoid cells. VI. Induction of DNA polymerases alpha, beta, and gamma following stimulation with phytohemagglutinin. Blood. 1975 Oct;46(4):509–518. [PubMed] [Google Scholar]
  16. Moss D. J., Pope J. H. EB Virus-associated nuclear antigen production and cell proliferation in adult peripheral blood leukocytes inoculated with the QIMR-WIL strain of EB virus. Int J Cancer. 1975 Mar 15;15(3):503–511. doi: 10.1002/ijc.2910150316. [DOI] [PubMed] [Google Scholar]
  17. Noy G. C., Dalpra L., Pedrini A. M., Ciarrocchi G., Giulotto E., Nuzzo F., Falaschi A. Evidence for two waves of induction of DNA enzymes in stimulated human lymphocytes. Nucleic Acids Res. 1974 Sep;1(9):1183–1199. doi: 10.1093/nar/1.9.1183. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Paterson M. C., Smith B. P., Lohman P. H., Anderson A. K., Fishman L. Defective excision repair of gamma-ray-damaged DNA in human (ataxia telangiectasia) fibroblasts. Nature. 1976 Apr 1;260(5550):444–447. doi: 10.1038/260444a0. [DOI] [PubMed] [Google Scholar]
  19. Prosser J. S. Survival of human T and B lymphocytes after X-irradiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1976 Nov;30(5):459–465. doi: 10.1080/09553007614551271. [DOI] [PubMed] [Google Scholar]
  20. Remsen J. F., Cerutti P. A. Deficiency of gamma-ray excision repair in skin fibroblasts from patients with Fanconi's anemia. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2419–2423. doi: 10.1073/pnas.73.7.2419. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rogers J. C. Identification of an intracellular precursor to DNA excreted by human lymphocytes. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3211–3215. doi: 10.1073/pnas.73.9.3211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Santos Mello R., Kwan D., Norman A. Chromosome aberrations and T-cell survival in human lymphocytes. Radiat Res. 1974 Dec;60(3):482–488. [PubMed] [Google Scholar]
  23. Scudiero D., Norin A., Karran P., Strauss B. DNA excision-repair deficiency of human peripheral blood lymphocytes treated with chemical carcinogens. Cancer Res. 1976 Apr;36(4):1397–1403. [PubMed] [Google Scholar]
  24. Setlow R. B., Regan J. D., German J., Carrier W. L. Evidence that xeroderma pigmentosum cells do not perform the first step in the repair of ultraviolet damage to their DNA. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1035–1041. doi: 10.1073/pnas.64.3.1035. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Smith C. A., Hanawalt P. C. Repair replication in human cells. Simplified determination utilizing hydroxyurea. Biochim Biophys Acta. 1976 May 19;432(3):336–347. doi: 10.1016/0005-2787(76)90143-x. [DOI] [PubMed] [Google Scholar]
  26. Spiegler P., Norman A. Kinetics of unscheduled DNA synthesis induced by ionizing radiation in human lymphocytes. Radiat Res. 1969 Aug;39(2):400–412. [PubMed] [Google Scholar]
  27. Sprent J., Anderson R. E., Miller J. F. Radiosensitivity of T and B lymphocytes. II. Effect of irradiation on response of T cells to alloantigens. Eur J Immunol. 1974 Mar;4(3):204–210. doi: 10.1002/eji.1830040310. [DOI] [PubMed] [Google Scholar]
  28. Stobo J. D., Paul W. E. Functional heterogeneity of murine lymphoid cells. 3. Differential responsiveness of T cells to phytohemagglutinin and concanavalin A as a probe for T cell subsets. J Immunol. 1973 Feb;110(2):362–375. [PubMed] [Google Scholar]
  29. Taylor A. M., Harnden D. G., Arlett C. F., Harcourt S. A., Lehmann A. R., Stevens S., Bridges B. A. Ataxia telangiectasia: a human mutation with abnormal radiation sensitivity. Nature. 1975 Dec 4;258(5534):427–429. doi: 10.1038/258427a0. [DOI] [PubMed] [Google Scholar]

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