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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: Free Radic Biol Med. 2011 Oct 7;52(2):427–435. doi: 10.1016/j.freeradbiomed.2011.09.020

Fig. 3.

Fig. 3

Assessment of PI3K and Akt inhibition effects upon GTN-induced blood pressure decreases in anesthetized rats. Experiments were performed using the tail-cuff method. Animals were anesthetized with ketamine/xylazine mixtures according to the Materials and methods. Blood pressure was stabilized for at least 20 min before GTN administration. Wortmannin (0.5 mg/kg) or Akt inhibitor (2 mg/kg) was administrated 2 h before the experiments in DMSO (50 µL injection volume). Controls received DMSO injection equivalent to the amount required for inhibitor treatment 2 h before GTN.