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. Author manuscript; available in PMC: 2012 Sep 2.
Published in final edited form as: Free Radic Biol Med. 2011 Oct 7;52(2):427–435. doi: 10.1016/j.freeradbiomed.2011.09.020

Fig. 4.

Fig. 4

PI3K/Akt-dependent phosphorylation of eNOS elicited by nitroglycerin. BAEC were challenged with nitroglycerin (500 nM) for 3 min before harvest. PI3K inhibitor wortmannin (100 nM) or Akt inhibitor (20 µM) was dissolved in DMSO and added to the cell cultures 1 h before GTN treatment; DMSO was also added at the same concentration (v/v) to control groups. Final DMSO concentration did not exceed 0.1%.