Figure 2.

Electrophoresis agarose gel of digested NAT1 PCR Products. Lane M is the marker phiX174 DNA/BsuRI (Fermentas). (a) Alw26I digestion products for C¹⁰⁹⁵A detection indicates that Lanes 1 and 5 do not possess the mutation, Lanes 2 and 4 are heterozygous mutants, and Lane 3 is a homozygous mutant. (b) HinfI digestion products for G⁵⁶⁰C detection indicate that Lanes 1, 3, and 4 do not possess the mutation, Lane 5 is heterozygous mutant, and Lane 2 is homozygous mutant. (c) AseI digestion products for T¹⁰⁸⁸A detection indicate that Lanes 1 and 4 do not possess the mutation, Lane 3 is heterozygous mutant, and Lanes 2 and 5 are homozygous mutants. (d) ScaI digestion products for C⁵⁵⁹T detection indicate that Lanes 1, 3, 4, and 5 do not possess the mutations, while Lane 2 is heterozygous mutant.