(a) PCR-RFLP primers, enzymes, and conditions for NAT1 genotyping

Detected mutation		Annealing T°C	Expected	Restriction
	Primers		fragment	digestion at
			size in bp	37°C
C1095 A	1095-F 5′-CAT TTC ACC TAT AAA AAT GTC-3′	58	132	Alw26I
	1095-r 5′-CCA AGA TAA CCA CAG GCC ATC TTT AAG A-3′
C559 T	Mt2-f 5′-ATC TCC TAG AAG ACA GCA AGT AC-3′	55	138	ScaI
	Mt2-r 5′-AGG AAC AAA ATG ATT TAC TA-3′
G560 C	SJ-f 5′-CTC CTG CAG GTG ACC ATT GAT-3′	55	261	HinfI
	SJ-r 5′-CTT AAG AGT AAA GGA GTA GAT GAT T-3′
	1088-f 5′-CAA ACC TTT TCA AAT AAT AAT AAT AAT AAT
T1088 A	ATT AA-3′	55	96	AseI
	1088-r 5′-GCT TTC TAG CAT AAA TCA CCA A-3′