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. Author manuscript; available in PMC: 2013 Aug 24.
Published in final edited form as: Mol Cell. 2012 Jul 19;47(4):570–584. doi: 10.1016/j.molcel.2012.06.014

Figure 1. PTEN down regulation and HER2 overexpression synergize to increase the CSC population in vitro.

Figure 1

(A and F) Down regulation of PTEN and/or overexpression of HER2 in MCF7 or Sum159 cells is demonstrated by western blotting. (B and G) PTEN down regulation in HER2 overexpressing cells increased the tumorsphere formation, (C and H) Aldefluor-positive cell populations (D and I) as well as invasion compared to either HER2 overexpression or PTEN down regulation in vitro. (E) MCF7-HER2+PTEN− cells showed a significant increase in the proportion of CD44+/CD24− cells as compared to the MCF7-HER2+ or MCF7PTEN− cells. Error bars represent the mean and standard deviation of two independent experiments performed in duplicate samples. (*p ≤ 0.05, # p ≤ 0.01)