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. 2012 Sep;181(3):993–1006. doi: 10.1016/j.ajpath.2012.05.028

Figure 2.

Figure 2

Effect of LOV on CYP24A1 and CYP27B1 expression in the spinal cords of EAE rats. Spinal cords were collected 16 dpi from EAE and control rats treated with LOV or vehicle as detailed in Figure 1 legend. A: Composite mean ± SEM of four to five samples per group of the levels of CYP24A1 and CYP27B1 transcripts analyzed by real-time PCR in the spinal cord tissues. B: Representative autoradiograph depicting the levels of CYP24A1, CYP27B1, and β-actin proteins analyzed by Western blotting in the spinal cord tissues. C: Histogram depicting the composite mean ± SEM of four to five samples per group of the levels of CYP24A1 and CYP27B1 proteins normalized to β-actin and compared with respective controls in the spinal cord tissues. Statistical significance as indicated: **P < 0.01; ***P < 0.001; NS, not significant.