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. 2012 Sep;181(3):775–784. doi: 10.1016/j.ajpath.2012.05.025

Figure 3.

Figure 3

Altered gene expression in lactating breast tissues from α-lactalbumin–immunized mice. Six weeks after immunization with either α-lactalbumin or CFA alone, total RNA from lactating mammary tissue was analyzed for gene expression by real-time qRT-PCR. A: Lactating breast tissue from α-lactalbumin–immunized mice showed significantly elevated gene expression levels of IFN-γ (P = 0.001) but not IL-10 (P > 0.10) and significantly increased gene expression of α-lactalbumin (P < 0.002) and α-casein (P = 0.0008). Asterisks indicate significance. B: α-Lactalbumin and α-casein gene expression levels were not elevated in nonlactating breast tissue from α-lactalbumin–immunized mice. C: Except for the spleen, IFN-γ gene expression levels were not elevated in any tissues examined from normal nonlactating α-lactalbumin–immunized mice. Data are given as mean ± SE.