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. 2012 Sep;181(3):845–857. doi: 10.1016/j.ajpath.2012.05.020

Figure 8.

Figure 8

Mast cell stabilizer cromolyn sodium exacerbates BAL eosinophil recruitment and mucin overproduction. A: Tryptase secretion. Mast cell stabilization 30 minutes postgavage was monitored by measuring a significant decrease in tryptase secretion into the BAL in cromolyn-treated mice. B: Mast cell staining. Mast cell stabilization was confirmed by counting toluidine blue–stained mast cells in ethanol-fixed lungs. A significant increase in granule-intact toluidine blue–stained mast cells was observed in cromolyn-treated mice. C: BAL eosinophils. Eosinophil infiltration in the BAL was quantified 30 minutes postgavage. Cromolyn treated mice exhibited a significant fourfold increase in BAL eosinophils compared to saline-treated controls. D: Representative PAS-stained histology showing mucin production in lungs of vehicle-treated ethanol-gavaged sensitized mice (left) and cromolyn-treated ethanol-gavaged sensitized mice (right). E: Morphometric analysis of histology at ×4 magnification from D shows a significant increase in mucin production in cromolyn-treated mice. Data are mean ± SEM, n = 6 to 7 per group. *P < 0.05, ***P < 0.001, using a priori one-tailed Student's t-test (A and B) and two-tailed Student's t-test (C and E).