Figure 1.

Naïve Mϕ-TGF-βRIIKO mice have reduced TGF-βRII expression on lung macrophages and develop pulmonary inflammation at baseline. A: Flow cytometry gating strategy for the identification of TGF-βRII expression on CD11c-positive cell populations in whole lung digests of naïve wild-type (WT) and Mϕ-TGF-βRIIKO mice. FSC^highSSC^low cells (R1) that coexpressed CD45 and CD68 (R2) were evaluated for surface expression of CD11c and TGF-βRII. In the contour plots, the percentage of positive cells is indicated in each quadrant. B: Mean fluorescence intensity (MFI) for TGF-βRII on the CD11c^low and CD11c^high populations. C: Results of a quantitative RT-PCR-based wound-healing array performed on whole lung tissues from naïve WT and Mϕ-TGF-βRIIKO strains. Data are expressed as means ± SEM (B) or from pooled samples representative of two independent experiments (C). **P < 0.01. n = 4 mice (A and B); n = 2 or 3 mice (C) per group.