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. 2012 Sep;181(3):897–906. doi: 10.1016/j.ajpath.2012.05.032

Figure 2.

Figure 2

Bone marrow-derived macrophages (BMDMs) from Mϕ-TGF-βRIIKO mice lack TGF-β–mediated suppression of antigen-specific T cell activation. A: Flow cytometry gating strategy for the identification of ovalbumin-specific TCR transgenic CD4+ lymphocytes (OTII) after coculture with BMDMs from WT or Mϕ-TGF-βRIIKO mice via coexpression of TCR-β and CD4. B: Representative contour plots of gated CD4+ T cells show the level of BrdU incorporation and cell surface expression of CD25 (IL-2Rα) at 96 hours. In the contour plots, the percentage of positive cells is indicated in each quadrant in contour plots; in the bar graphs, the percentage of BrdU-positive cells in the CD25− or CD25+ gate is shown. APC-CD4+ T-cell cocultures were administered medium only (Mock), 20 ng/mL recombinant murine TGF-β (TGF-β), 1 mg/mL OVA peptide (OVA), or TGF-β and OVA peptide. Data are expressed as means ± SEM of quadruplicate wells and are representative of two independent experiments. *P < 0.05; ***P < 0.001. n = 4 mice per group. n.s., not significant.