Figure 2.

A–D: Comparison of mean values for each tympanometry parameter over a time course. At least 6 wild-type (+/+) and 6 Lmna^Dhe/+ mutant (Dhe/+) mice were assessed at each time point. E and F: Scanning tympanograms from wild-type and mutant mice at 5 months of age present, respectively, the normal A type curve (E, tall narrow curve with the peak near 0 daPa pressure) and the abnormal C type curve (F, low wide curve with the peak residing at negative pressure). G and H: Anatomical images under otoscopy of the same ears assessed in E and F, respectively. In the mutant mouse ear (H), tympanic membrane adherence (asterisk) was visible, and hyperemia (white arrow), hydrotympanum, and a shortened light cone (black arrow) were detected. A representative wild-type mouse ear (G) displays a normal tympanic membrane with a subuliform light cone on its anteroinferior quadrant (black arrow). Data are expressed as means ± SD. *P < 0.05; **P < 0.01. Scale bar = 500 μm.