Figure 9.

Detection of nuclear lamina morphology and expression disparity of TGF-β and TNF-α in peritoneal macrophages of wild-type (+/+) and Lmna^Dhe/+ mutant (Dhe/+) mice. Shown are representative images of immunofluorescent staining of peritoneal macrophages of wild-type and mutant mice. A–D: Staining with anti-LMNA antibody reveals nuclear lamina morphology in peritoneal macrophages. Note the compact, plump, and even meshwork of the nuclear envelope, apparent as green fluorescence (arrows) in wild-type cells (A), compared with the rugous, disintegrative or even collapsed envelope (arrows) in Dhe/+ cells (C). Nuclei were larger in mutant than in wild-type cells. B and D: Nuclear localization was confirmed by counterstaining with propidium iodide (red). E–L: Peritoneal macrophages were stained with anti-TGF-β (E–H) or anti-TNF-α (I–L) antibody, revealing expression of these proteins in mutant macrophages, but with only faint staining (if any) in those from wild-type macrophages. Again, macrophages exhibited enlargement in the mutant mice, and expression of both TGF-β and TNF-α was observed in both the cytoplasm and the nucleus (arrows). F, H, J, and L: Merged images corresponding to images E, G, I, and K, respectively. DAPI counterstain (blue; counterstain images not shown separately) was used to reveal nuclei. Scale bar = 10 μm.