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. 2012 Sep;181(3):866–874. doi: 10.1016/j.ajpath.2012.05.036

Figure 1.

Figure 1

Nutlin-3 prevents both the RV-induced decline in the level of p53 and contraction. A: The impact of Nutlin-3 on RV-mediated signaling events. RCFs are pretreated with either Nutlin-3 (10 μmol/L) or vehicle for 30 minutes and then exposed to either RV (diluted 1:3 in DMEM) or DMEM for 2 hours. The resulting cell lysates are subjected to Western blot analysis using the indicated antibodies. The numbers are a ratio of p- PDGFRα/PDGFRα, p-Akt/Akt, or p53/RasGAP. The data presented are representative of three independent experiments. B: The impact of Nutlin-3 on RV-mediated contraction. RCFs are subjected to the collagen contraction assay. Nutlin-3 (10 μmol/L) or vehicle was added as indicated. The data are subjected to a paired t-test. The asterisk indicates a statistically significant difference.