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. 2012 Sep;181(3):866–874. doi: 10.1016/j.ajpath.2012.05.036

Figure 2.

Figure 2

Nutlin-3 prevents retinal detachment and elevates p53 in epiretinal membranes. A: The impact of Nutlin-3 on experimental PVR. RCFs are used to induce PVR, as described in Materials and Methods. The indicated rabbits receive no additional injections (none) or 0.1-mL injections of either vehicle or Nutlin-3 (20 μmol/L) on days 0, 2, and 4. PVR was graded according to the Fastenberg scale of classification 41: stage 0, no disease; stage 1, epiretinal membrane; stage 2, vitreoretinal traction without retinal detachment; stage 3, localized retinal detachment (1 to 2 quadrants); stage 4, extensive retinal detachment (2 to 4 quadrants without complete detachment); stage 5, complete retinal detachment. The data are subjected to an unpaired t-test or a one-way analysis of variance test. The asterisk denotes a statistically significant difference. B: Nutlin-3 enhances expression of p53 in epiretinal membranes. At the end of the experiment (day 28), the eyes are enucleated and paraffin sections of whole eyes are subjected to p53 IHC. Arrows, epiretinal membrane; arrowheads, retina; brown, p53. Left and right panels: A Nutlin-3–injected animal. Middle panel: A rabbit treated with vehicle. Left and middle panels: Stained with the p53 antibody. Right panel: Stained with non–immune IgG. Scale bar = 50 μm.