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. 2012 Aug 21;3:1016. doi: 10.1038/ncomms2016

Figure 3. S402A mutation partly inhibits the autophosphorylation of PINK1.

Figure 3

(a) The S402A mutation altered the phosphorylation pattern of PINK1. PINK1 with the indicated mutations were transfected into HeLa cells and then subjected to SDS–PAGE on a 7.5% Tris-glycine gel ±50 μM phos-tag. Asterisks indicate phosphorylated PINK1 (see details of red and black asterisks in the text). (b) Ser/Thr residues in the activation loop of PINK1 from various organisms are shown in red font. Ser402 (boxed) is evolutionarily conserved.