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. 2012 Aug 28;3:1027. doi: 10.1038/ncomms2024

Figure 6. Multimode fibre-based fluorescence microscopy.

Figure 6

The sample contains fluorescent polymer particles having 2 μm in diameter. The top row (a–f) shows images sample volume directly imaged onto a CCD detector (see Fig. 6). Excitation signal in the form of 11×11 matrix of focal points is clearly visible. The bottom row (g–l) are images reconstructed from the fluorescence signal detected by a PMT (Fig. 6) behind the optical fibre. Each column shows the imaging performance for different resolution and frame rates. (a,g) Imaging for one static hologram at the SLM giving image resolution of 11×11 data points with 32 frames per second. (b,h) The SLM cycles between four different holograms to increase the resolution to 22×22 data points at the expense of frame rate, reduced to 7.7 Hz. (c,i) The SLM cycles between nine different holograms leading to 33×33 data points and frame rate of 3.8 Hz. (d,j) The SLM cycles between 16 holograms leading to 44×44 data points and frame rate of 2.2 Hz. (e,k) The SLM cycles between 25 holograms leading to 55×55 data points and frame rate of 1.4 Hz. (f,l) The SLM cycles between 36 holograms leading to 66×66 data points and frame rate of 1.0 Hz.