Fig. 5.
Effect of egg wax in influenza virus (H1N1) replication. Culture of MDCK cells, performed in 96 wells microplate, were infected with 128 UHA of influenza virus (A and B). In B, crude A. cajennense eggs wax extract with different concentrations (800, 400, 200, 100, 50, 25 and 12.5 μg/mL) was added 1 h before. After 72 h post infection cytopathic effect induced by the virus was observed. The medium of culture was removed and the cells in the plates were stained with crystal violet. Cells without cytopathic effect stained in blue