Fig. 5.

Adipogenic and osteogenic differentiation of c-kit⁺ and c-kit⁻ AFS cells. aOil-Red O staining for lipid vacuoles accumulation after adipogenic differentiation in c-kit⁺ AFS cells (a) and c-kit⁻ AFS cells (b). Von Kossa staining for mineralization after osteogenic differentiation in c-kit⁺ AFS cells (c) and c-kit⁻ AFS cells (d), b RT-PCR for adiponectin and osteopontin expression after adipogenic and osteogenic differentiation. Adiponectin (187 bp): 1 c-kit⁺; 2 c-kit⁻; osteopontin (173 bp): 3 c-kit⁺; 4 c-kit⁻. (Color figure online)