Figure 2. Dectin-1 regulates severity of colitis.

Wild type (WT) and Clec7a^−/− littermates were treated with 2.5% DSS for 7 days and kept on water for 4 additional days. Colitis progress and severity were assessed by measuring body weight during treatment (A) and histology (B, C), and TNF-α production in the colon (D) on day 11. (E, F) WT and Clec7a^−/− mice were given an antibiotic cocktail including fluconazole for 3 weeks, transplanted as indicated (red) with fecal microflora from WT or Clec7a^−/− mice and treated with DSS as in (A). Disease severity was accessed by histology score (E) and by cytokine production by anti-CD3/anti-CD28 stimulated LI-LP and MLN T cells (F). Each symbol represents a different mouse. One of four independent experiments is shown. Error bars, s.d., * P < 0.05, ** P < 0.01.