Figure 1.

Master curve generated by plotting the mean Cq values as a function of the known starting concentration of the standard dilutions. Ten-fold serial dilutions (10⁶ - 1 copy) of the linearized T. annulata recombinant plasmid were analyzed in 9 independent experiments with three replicate reactions per dilution step. Error bars represent standard deviations (SD) of Cq values from replicated standards. Confidence intervals (CI) of the means of Cq values from replicated standards are indicated by the dashed lines.