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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jul 3;1820(11):1734–1743. doi: 10.1016/j.bbagen.2012.06.020

Figure 3. TM-PCI causes an increase in the cytosolic concentration of calcium.

Figure 3

A) Images of HeLa cells incubated with TMR-TAT and the calcium indicator fluo-4AM. The fluo-4 images are pseudocolored based on the fluorescence intensity of the probe. Images representing the distribution of TMR-TAT before and after light irradiation are presented as inverted monochromes. B) Intensity of the fluo-4 signal as a function of light exposure time (irradiation at 560 nm). Cells were incubated with fluo-4AM alone, or with TMR-TAT (3 μM) in L-15 containing 1.3 mM CaCl2. Cells were then placed in fresh medium containing 0 or 1.3 mM CaCl2. Cells were irradiated immediately so as to prevent depletion of calcium intracellular stores for the cells lacking calcium in the extracellular milieu.