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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jul 3;1820(11):1734–1743. doi: 10.1016/j.bbagen.2012.06.020

Figure 6. TMR-TAT co-localizes with endocytic organelles that contain calcium and TM-PCI causes the release of the content of endocytic organelles.

Figure 6

A) Microscopy images showing that TMR-TAT (pseudo-colored red) co-localizes with intracellular organelles expressing Cerulean-LAMP1 (pseudo-colored green). Upon irradiation, the TMR-TAT signal decreases from certain organelles as indicated by the white arrows. B) TMR-TAT co-localizes with endocytic organelles that contain calcium. HeLa cells were incubated with TMR-TAT and the cell-impermeant calcium probe fura-2. Ratiometric imaging of fura-2 was performed by measuring the emission of the probe at 505 nm after excitation at 340 nm or 380 nm. C) TM-PCI causes the release of the pinocytosis marker 3 kDa Dextran Rhodamine green (Dx-RG). Representative images of HeLa cells incubated with TMR-TAT and RG-Dx before and after light irradiation at 560 nm. The overlay image of the TMR (pseudo-colored red) and RG (pseudo-colored green) shows a punctate distribution for both species prior to irradiation (a yellow color being indicative of co-localization). After irradiation, the fluorescent signals of TMR-TAT and Dx-RG are distributed throughout the cell. This does not happen if TMR-TAT is not present (not shown).