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Levels of glucagon mRNA are affected by visual experience in WT and RGE retinas. Quantitative RT-PCR was used to measure total retinal levels of pro-glucagon mRNA. Levels were standardized to GAPDH. Retinas were harvested from eyes that were treated with 4.5 days of form-deprivation (n=5), minus lens-wear (n=5), or plus lens-wear (n=4). Significance of difference was determined by using a two-tailed Student’s t-test.
