Table 1.

experimental paradigms

Experiment # and Figure #	Numbers of animals	Treatment	Outcome measures
Experiment 1 Figure 1	10 WT 9 RGE	6 consecutive days of FD	Digital biometry of enucleated eyes
Experiment 2 Figure 2	10 WT 10 RGE	4.5 consecutive days of Plus-lens wear	Refraction, ultrasound, and digital biometry of eyes
	10 WT 10 RGE	4.5 consecutive days of Minus-lens wear	Refraction, ultrasound, and digital biometry of eyes
	6 WT 6 RGE	4.5 consecutive days of Plano-lens wear	Refraction, ultrasound, and digital biometry of eyes
Experiment 3 Figure 3	10 WT 12 RGE	7 consecutive days of FD- uninterrupted	Refraction, ultrasound, and digital biometry of eyes
	12 WT 14 RGE	7 days of FD interrupted daily by 130 minutes of unrestricted vision	Refraction, ultrasound, and digital biometry of eyes
Experiment 4 Figures 4a-d	5 WT 5 RGE	6 consecutive days of FD followed by 2 hrs of unrestricted vision	Retinas processed for Egr1/glucagon immunofluoresence
	5 WT 5 RGE	4.5 days of minus lens-wear followed by 2 hrs of unrestricted vision	Retinas processed for Egr1/glucagon immunofluoresence
	5 WT 5 RGE	4.5 days of plus lens-wear followed by 2 hrs of unrestricted vision	Retinas processed for Egr1/glucagon immunofluoresence
Experiment 5 Figures 4e-h	6 WT 5 RGE	2 hrs of minus lens-wear	Retinas processed for Egr1/glucagon immunofluoresence
	6 WT 5 RGE	2 hrs of plus lens-wear	Retinas processed for Egr1/glucagon immunofluoresence
Experiment 6 Figure 5	5 WT 5 RGE	4.5 days FD	Retinas individually processed for mRNA extraction, cDNA synthesis and qRT-PCR
	5 WT 5 RGE	4.5 days of minus lens-wear	Retinas individually processed for mRNA extraction, cDNA synthesis and qRT-PCR
	4 WT 4 RGE	4.5 days of plus lens-wear	Retinas individually processed for mRNA extraction, cDNA synthesis and qRT-PCR

Abbreviations: WT – wild type, RGE – retinopathy, globe enlargement, FD- form-deprivation, qRT-PCR - quantitative reverse transcriptase polymerase chain reaction.