Skip to main content
. Author manuscript; available in PMC: 2013 Oct 31.
Published in final edited form as: J Immunol Methods. 2012 Jul 20;384(1-2):43–50. doi: 10.1016/j.jim.2012.07.003

Figure 6.

Figure 6

Fucosidase treatment of sLex microspheres had a slight inhibitory effect on HECA-452 recognition of sLex microspheres but a relatively robust inhibitory effect on sLex microsphere adhesion to E-selectin. (A) Levels of HECA-452 reactive epitopes on sLex microspheres, with and without fucosidase treatment, were analyzed by flow cytometry. Top panels - microspheres prepared with no sLex and not treated with fucosidase; Middle panels - microspheres prepared with 0.125 μg/ml sLex and not treated with fucosidase; Bottom panels - microspheres prepared with sLex and treated with fucosidase (0.1 U/ml). (B) SLex microspheres, treated with and without fucosidase 24 hr prior to the adhesion assay, were perfused over 4 hr. IL-1β activated HUVEC at a shear stress of 1.5 dynes/cm2. F indicates fucosidase treatment; *p≤0.05; n ≥4.