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. Author manuscript; available in PMC: 2013 Oct 31.
Published in final edited form as: J Immunol Methods. 2012 Jul 20;384(1-2):43–50. doi: 10.1016/j.jim.2012.07.003

Figure 7.

Figure 7

HECA-452 does not block neutrophil accumulation on 4 hr. IL-1β activated HUVEC. Freshly isolated human neutrophils were untreated, treated with rat IgM or treated with 100 μg/ml HECA-452, as indicated, prior to the adhesion assay and perfused over 4 h. IL-1β activated HUVEC for 2.5 minutes. The number of adherent neutrophils was determined after the perfusion period and normalized to the area of the field of view. Shear stress = 1.5 dynes/cm2; Results shown are the average of n=3.