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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 16;1823(10):1796–1806. doi: 10.1016/j.bbamcr.2012.06.010

Figure 1.

Figure 1

The PMA- and A23187-treated type II cells show increased co-localization of annexin A7 (A7) with ABCA3 or with SNAP23. Adherent cells were treated for indicated periods without or with 250 nM A23187 or 80 nM PMA, fixed and stained A7 and ABCA3 (A) or for SNAP23 and biotinylated anti-A7 (B). The pixel intensities for individual fluorophore for laser confocal microscope (LSM) images were adjusted to the same range for all groups. Cell treatment with PMA or A23187 increases co-localization of A7 with ABCA3 and with SNAP23 in a time-dependent manner. Weighted co-localization coefficients (fractional intensity of co-localizing pixels relative to total pixel intensity) for individual fluorophore are shown for each condition. Representative images are shown from experiments that were repeated at least two times.