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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Jun 16;1823(10):1796–1806. doi: 10.1016/j.bbamcr.2012.06.010

Figure 4.

Figure 4

Secretagogue-mediated increase in co-localization of ABCA3 with SNAP23 is blocked by PKC inhibitor. Isolated type II cells were treated for 30 min without or with 100 nM BisI prior to 30 min incubation with 80 nM PMA or 250 nM A23187. The cells were fixed and stained for SNAP23 and ABCA3 and the nuclei were counter-stained with DAPI. A. Normalized images for green (SNAP23) and red (ABCA3) fluorophores show that BisI pretreatment diminishes secretagogue-mediated increases in co-localization of two proteins. The co-localization patterns and weighted co-localization co-efficient (CC) for individual fluorophore are shown in the lower panels for each image. B. Weighted CC from 4 - 6 experiments are plotted as mean ± SE. ** P < 0.05 in comparison to the controls (ANOVA) and * P < 0.05 in comparison to without BisI (Student’s t-test).