Figure 2.

SLOB cycling is altered under DD conditions in clock mutants. (A) Western blot analysis of SLOB cycling in Canton S, per⁰¹, tim⁰¹ and Clk^jrk flies under LD conditions. Oscillations in all flies are in phase with one another. The graphs represent the mean ± SEM for each time point from a minimum of three experiments for each genotype. (B, C) Western blot analysis of Canton S, Clk^jrk and per⁰¹ flies in DD conditions. There is no obvious cycling of SLOB in Clk^jrk and per⁰¹ flies. (D) Western blot analysis of Canton S and tim⁰¹ flies in DD conditions. There is a shift in the phase of SLOB cycling in tim⁰¹ flies. The peak is no longer at CT 14 but at CT 2. The same Canton S data are illustrated in panels (B-D).