Figure 7.

SLOB expression is altered in Clk^jrk and Pdf⁰¹ flies. (A) Brain wholemount from wild type rosy (ry) and Clk^jrk flies. Two time points, taken on the second day of DD, show that SLOB does not cycle in the PI neurons of the Clk^jrk flies. The accompanying bar graph illustrates relative intensity scores. Data were analyzed as described in Figure 5. (B) Brain wholemount from wild type y w and Pdf⁰¹ flies. Two time points, taken on the second day of DD, demonstrate the decrease in SLOB expression and dampened cycling in the PI neurons of Pdf⁰¹ flies. PDF appears to positively regulate SLOB protein. The bar graph represents relative intensity scores.