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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Cell Signal. 2012 Jul 1;24(11):2051–2060. doi: 10.1016/j.cellsig.2012.06.011

Figure 2. D4DR-mediated regulation of type I and type II AC activity.

Figure 2

HEK 293 cells were transfected so that they expressed β2AR and D4DR with or without either type I AC or type II AC-RLuc. Panel a: Cells were then assayed for cAMP accumulation in the absence (Basal) or in the presence of dopamine (DA) as described in Materials and Methods. Panel b: Data in panel (a) are shown together with the response of cells to isoproterenol (Iso) or both Iso and DA together (Iso + DA). Data in panel (b) are shown on a different scale than the data in panel (a). Basal levels of cAMP accumulation in the absence of exogenously expressed AC (No Exogenous AC) were not significantly affected by dopamine (p>.05, n=3). Significant differences based on the indicated number (n) of samples from three independent experiments were determined with GraphPad Prism 4 software (** p<0.01).