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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Cell Signal. 2012 Jul 1;24(11):2051–2060. doi: 10.1016/j.cellsig.2012.06.011

Figure 3. BRET between β2AR-RLuc and tetracysteine-tagged D4DR.

Figure 3

HEK 293 cells co-expressing β2AR-RLuc, Gαs, Gαi1, Gβ1, Gγ2, AC and the indicated tetracysteine-tagged D4DR were incubated with FlAsH and BRET was assayed following the addition of coelenterazine h. The effects of isoproterenol (Iso) or dopamine (DA) either individually or together on BRET were determined as described in Materials and Methods. The BRET background was determined in cells co-expressing D4DR that lacked a tetracysteine tag and either β2AR-RLuc or RLuc. There was a significant difference between the background BRET and BRET in cells expressing D4DR-G259C or D4DR-G275C (p<0.05) but not for cells expressing the D4DR-PGCC construct (p>0.05). There were no significant differences (p>0.05) between BRET in the presence and absence of agonists for a particular D4DR construct. The data are representative of five independent experiments.