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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Cell Signal. 2012 Jul 1;24(11):2051–2060. doi: 10.1016/j.cellsig.2012.06.011

Figure 5. BRET between Gαi1-RLuc donors and tetracysteine-tagged D4DR.

Figure 5

HEK 293 cells co-expressing β2AR, Gαs, Gβ1, Gγ2, AC and the indicated combination of tetracystein-tagged D4DR and Gαi1-RLuc were treated as described in Fig. 3. The BRET background was determined in cells co-expressing D4DR that lacked a tetracysteine tag. The data is representative of five independent experiments.