Figure 3.

Deletion of cdhA results in the accumulation of CB in subapical cells at 25°C. (A) The arrows in the brightfield images indicate septa, showing the boundaries of each cell and revealing that each cell is subapical (i.e., has no hyphal apex). In the cdhA⁺ strain, CB-GFP did not accumulate in this cell or other subapical cells, but in the cdhAΔ strain, it did accumulate. CB accumulated at SPBs in the cdhAΔ strain (arrowheads; brightfield images are single focal plane, and others are projections). (B) Quantitation of the accumulation of CB-GFP in subapical cells of cdhA⁺ and cdhAΔ strains (mean of three experiments for each strain; error bars are SDs; n = 90 for cdhA⁺; n = 80 for cdhAΔ).