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. 2012 Sep 3;198(5):833–846. doi: 10.1083/jcb.201204102

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© 2012 Elhanany-Tamir et al.

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Figure 4. — The astral MTs detach from the myonuclei in msp-300 and klar mutants and overlap the MSP-300 nuclear ring. Striated larval muscles of wild type (A, A′, and A″), klar^Δ1–18 (B, B′, and B″), msp-300^ΔKASH (C, C′, and C″), msp-300^compl/+;klar^Δ1-18/+ double heterozygous (D, D′, and D″), and msp-300^Δ3′ (E, E′, and E″) mutants labeled with MSP-300 (green; A, A″, B, B″, C, C″, D, D″, E, and E″), α-Tubulin (red; all panels), and lamin (blue; A, A′, B, B′, C, C′, D, D′, E, and E′). Arrowheads in all panels indicate the overlap between α-Tub staining and the MSP-300 nuclear ring. Note the complete disruption of the astral MT in msp-300^Δ3′ mutant. Bar, 10 µm.