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. 2012 Sep 3;198(5):815–832. doi: 10.1083/jcb.201201050

Figure 1.

Figure 1.

Satellite cells express Six1. (A) qRT-PCR analysis indicated expression of SIX family transcripts by freshly FACS-sorted SCs (Satellites), myogenic cells cultured in growth medium (Myoblasts), or induced to differentiate by serum removal for 3 d (Myotubes). Error bars indicate standard deviations. (B) Single myofibers isolated from EDL muscles of C57BL/6 mice. Myofibers were cultured in floating conditions and immunolocalized for Six1 and Pax7 or Myogenin proteins at different times after isolation. All quiescent, dividing, or differentiating SCs expressed Six1. Bars, 10 µm.